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Multicellular indicator organisms is their presence a good sign?

1/2/2018

 
Microscopic exam is a good way to survey biomass health in suspended growth systems. Conventional and extended aeration activated sludge systems are designed to operate at decline to endogenous phase on the growth curve. Protozoa indicative of good operating conditions include free-swimming ciliates, crawling ciliates, and stalk ciliates/suctorians. All of these protozoa are single cell organisms associated with floc formation and sufficient dissolved oxygen. As we move further into endogenous phase - lower F/M and longer MCRT - we start to see multicellular organisms in the MLSS. The most common being rotifers - these organisms are very common in large lagoon systems, fixed film, and activated sludge with low loadings. As we increase populations of rotifers and start seeing worms, tardigrades, and gastrotrichs, the polymers holding the floc together start to "digest" inside the aeration basin. In effect, the aeration basin becomes an aerobic digester. The multicellular indicators need higher dissolved oxygen and lower F/M conditions than the single cell protozoa - and thrive in extreme low F/M conditions. So what can be the problem when multicellular organisms predominate over protozoa? 
  • Floc begins to fragment - smaller floc
  • "Fines" begin to be seen in the water column and on top of the clarifier
  • Effluent turbidity increases 
  • Polymer demand increases to prevent solids carryover
  • Viable portion of the biomass decreases as MLSS becomes more "dead" biomass and particulates
The above conditions indicate "old sludge" and the solution is to increase wasting. Lowering MLSS increases the F/M, lowers MCRT - which increases EPS (biological polymers) and number of viable bacteria.

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    Author

    Erik Rumbaugh has been involved in biological waste treatment for over 20 years. He has worked with industrial and municipal wastewater  facilities to ensure optimal performance of their treatment systems. He is a founder of Aster Bio (www.asterbio.com) specializing in biological waste treatment.

    View my profile on LinkedIn

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