Here is what I often see:
- Nocardia and other slow growing organisms that exploit a niche can become more of a problem
- Effluent turbidity and fines/pin-floc passing into effluent increase
- Beneficial EPS decreases - organisms store excess organics in EPS but as you enter endogenous respiration the bacteria begin to utilize EPS for cell maintenance/growth
- Oxygen requirements - while individual cell respiration rates drop with a mature biomass, the overall oxygen consumed is higher when you add up all the biological solids
- Filaments become more of a bulking agent with decreased floc former EPS